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Antibacterial Activity of Aqueous, Ethanol and Acetone Extracts of Ocimum sanctum Linn

Received: 24 October 2015     Accepted: 2 November 2015     Published: 17 November 2015
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Abstract

Aqueous, ethanol and acetone leaf and root extracts of Ocimum sanctum Linn were investigated for their antibacterial activities at various concentrations against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa using standard methods. Antimicrobial studies indicated that both the acetone leaf and root extracts of O. sanctum were found comparatively more effective against these bacteria than any other extract tested while aqueous extract being the least effective against the tested microbes. The highest mean zone of inhibition of acetone leaf extract against P. aeruginosa was 20.74± 0.68 followed by 19.36± 0.29 at concentration 150mg/ml and 100 mg/ml respectively. The aqueous and ethanol root extracts of O. sanctum for had no inhibitory effect against the test microorganisms. Generally the acetone crude extracts showed activity against the three bacteria species with highest average zone of inhibition compared to other extracts. The antimicrobial activity of the leaf extract was more pronounced against test microbes than root extract. The study revealed that the plant possessed antimicrobial properties and could be a potential source of antibacterial agent in the treatment of bacterial infections.

Published in American Journal of BioScience (Volume 3, Issue 6)
DOI 10.11648/j.ajbio.20150306.18
Page(s) 256-261
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Antibacterial Activities, Extract, Inhibition, Medicinal Plant, Ocimum sanctum

References
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  • APA Style

    Naima S. Seleman, Ezekiel Amri. (2015). Antibacterial Activity of Aqueous, Ethanol and Acetone Extracts of Ocimum sanctum Linn. American Journal of BioScience, 3(6), 256-261. https://doi.org/10.11648/j.ajbio.20150306.18

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    ACS Style

    Naima S. Seleman; Ezekiel Amri. Antibacterial Activity of Aqueous, Ethanol and Acetone Extracts of Ocimum sanctum Linn. Am. J. BioScience 2015, 3(6), 256-261. doi: 10.11648/j.ajbio.20150306.18

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    AMA Style

    Naima S. Seleman, Ezekiel Amri. Antibacterial Activity of Aqueous, Ethanol and Acetone Extracts of Ocimum sanctum Linn. Am J BioScience. 2015;3(6):256-261. doi: 10.11648/j.ajbio.20150306.18

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  • @article{10.11648/j.ajbio.20150306.18,
      author = {Naima S. Seleman and Ezekiel Amri},
      title = {Antibacterial Activity of Aqueous, Ethanol and Acetone Extracts of Ocimum sanctum Linn},
      journal = {American Journal of BioScience},
      volume = {3},
      number = {6},
      pages = {256-261},
      doi = {10.11648/j.ajbio.20150306.18},
      url = {https://doi.org/10.11648/j.ajbio.20150306.18},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20150306.18},
      abstract = {Aqueous, ethanol and acetone leaf and root extracts of Ocimum sanctum Linn were investigated for their antibacterial activities at various concentrations against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa using standard methods. Antimicrobial studies indicated that both the acetone leaf and root extracts of O. sanctum were found comparatively more effective against these bacteria than any other extract tested while aqueous extract being the least effective against the tested microbes. The highest mean zone of inhibition of acetone leaf extract against P. aeruginosa was 20.74± 0.68 followed by 19.36± 0.29 at concentration 150mg/ml and 100 mg/ml respectively. The aqueous and ethanol root extracts of O. sanctum for had no inhibitory effect against the test microorganisms. Generally the acetone crude extracts showed activity against the three bacteria species with highest average zone of inhibition compared to other extracts. The antimicrobial activity of the leaf extract was more pronounced against test microbes than root extract. The study revealed that the plant possessed antimicrobial properties and could be a potential source of antibacterial agent in the treatment of bacterial infections.},
     year = {2015}
    }
    

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    T1  - Antibacterial Activity of Aqueous, Ethanol and Acetone Extracts of Ocimum sanctum Linn
    AU  - Naima S. Seleman
    AU  - Ezekiel Amri
    Y1  - 2015/11/17
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajbio.20150306.18
    DO  - 10.11648/j.ajbio.20150306.18
    T2  - American Journal of BioScience
    JF  - American Journal of BioScience
    JO  - American Journal of BioScience
    SP  - 256
    EP  - 261
    PB  - Science Publishing Group
    SN  - 2330-0167
    UR  - https://doi.org/10.11648/j.ajbio.20150306.18
    AB  - Aqueous, ethanol and acetone leaf and root extracts of Ocimum sanctum Linn were investigated for their antibacterial activities at various concentrations against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa using standard methods. Antimicrobial studies indicated that both the acetone leaf and root extracts of O. sanctum were found comparatively more effective against these bacteria than any other extract tested while aqueous extract being the least effective against the tested microbes. The highest mean zone of inhibition of acetone leaf extract against P. aeruginosa was 20.74± 0.68 followed by 19.36± 0.29 at concentration 150mg/ml and 100 mg/ml respectively. The aqueous and ethanol root extracts of O. sanctum for had no inhibitory effect against the test microorganisms. Generally the acetone crude extracts showed activity against the three bacteria species with highest average zone of inhibition compared to other extracts. The antimicrobial activity of the leaf extract was more pronounced against test microbes than root extract. The study revealed that the plant possessed antimicrobial properties and could be a potential source of antibacterial agent in the treatment of bacterial infections.
    VL  - 3
    IS  - 6
    ER  - 

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Author Information
  • Department of Science and Laboratory Technology, Dar es Salaam Institute of Technology, Dar es Salaam, Tanzania

  • Department of Science and Laboratory Technology, Dar es Salaam Institute of Technology, Dar es Salaam, Tanzania

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