Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC

Ehab Abd El Atti; Alaa Dawood; Abdallah Said Essa; Bassam Mohamed Masoud; Enas Said Essa; Yasser El-Ghobashy; Ashraf Anas Zytoon

doi:doi:10.11648/j.ejpm.20130103.13

Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC

Ehab Abd El Atti, Alaa Dawood, Abdallah Said Essa, Bassam Mohamed Masoud, Enas Said Essa, Yasser El-Ghobashy, Ashraf Anas Zytoon

Published in European Journal of Preventive Medicine (Volume 1, Issue 3)

Received: 11 December 2013 Published: 10 January 2014

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Abstract

Background & Aim: Chronic HCV infection suppresses hepatic hepcidin expression which may enhance iron toxicity and lead to disease progression and HCC development. The aim of the study is to investigate the role of hepcidin in HCV+ve liver cirrhosis patients in relation to disease progression and HCC development. Patients and methods: The study population consists of: 20 HCV+ve patients without HCC (HCV patients), 20 HCV+ve patients with HCC (HCV-HCC patients) and 10 controls. In addition to comprehensive clinical examination, they were subjected to laboratory check-up for albumin, bilirubin, PT %, ferritin, AFP and hepcidin. Ascitic fluid hepcidin was done for all patients. Results: There was a significant difference among HCV and HCV-HCC patients and controls with regard serum ferritin and hepcidin (P = 0.001 & 0.0001 respectively). Serum hepcidin of HCV and HCV-HCC patients were significantly lower than controls (P = 0.0001). Serum and ascitic fluid hepcidin of HCV-HCC patients was significantly lower than HCV patients (P = 0.01 & 0.02 respectively). Serum ferritin was significantly higher in HCV and HCV-HCC patients than controls (P = 0.001). Serum ferritin of HCV-HCC patients was significantly higher than HCV patients (P = 0.02). Ascitic fluid hepcidin was negatively correlated with Child-Pugh score in HCV (r=-0.55 & P=0.01) and HCV-HCC patients (r = -0.53 & P = 0.02). Ascitic fluid hepcidin was negatively correlated with bilirubin in HCV (r = -0.43 & P=0.04) and HCV-HCC patients (r = -0.47 & P=0.04). Ascitic fluid hepcidin was positively correlated with serum albumin in HCV (r = +0.44 & P=0.04) but there was no correlation in HCV-HCC patients (r =-0.1 & P=0.7). Conclusion: Low levels of hepcidin may be involved in the pathophysiologic mechanism of iron overload in patients with chronic HCV with and without HCC. Moreover, there is a positive relationship between hepcidin levels and synthetic liver function suggesting that uniform suppression of hepcidin may be linked to disease progression and HCC development. Further analysis is still required to evaluate its usefulness as a marker for early detection of HCC by serial measurement of hepcidin in blood and ascitic fluid.

Published in	European Journal of Preventive Medicine (Volume 1, Issue 3)
DOI	10.11648/j.ejpm.20130103.13
Page(s)	63-69
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Hepcidin, HCC, HCV, Ferritin

References

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Ehab Abd El Atti, Alaa Dawood, Abdallah Said Essa, Bassam Mohamed Masoud, Enas Said Essa, et al. (2014). Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC. European Journal of Preventive Medicine, 1(3), 63-69. https://doi.org/10.11648/j.ejpm.20130103.13

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ACS Style

Ehab Abd El Atti; Alaa Dawood; Abdallah Said Essa; Bassam Mohamed Masoud; Enas Said Essa, et al. Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC. Eur. J. Prev. Med. 2014, 1(3), 63-69. doi: 10.11648/j.ejpm.20130103.13

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AMA Style

Ehab Abd El Atti, Alaa Dawood, Abdallah Said Essa, Bassam Mohamed Masoud, Enas Said Essa, et al. Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC. Eur J Prev Med. 2014;1(3):63-69. doi: 10.11648/j.ejpm.20130103.13

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@article{10.11648/j.ejpm.20130103.13,
  author = {Ehab Abd El Atti and Alaa Dawood and Abdallah Said Essa and Bassam Mohamed Masoud and Enas Said Essa and Yasser El-Ghobashy and Ashraf Anas Zytoon},
  title = {Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC},
  journal = {European Journal of Preventive Medicine},
  volume = {1},
  number = {3},
  pages = {63-69},
  doi = {10.11648/j.ejpm.20130103.13},
  url = {https://doi.org/10.11648/j.ejpm.20130103.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejpm.20130103.13},
  abstract = {Background & Aim: Chronic HCV infection suppresses hepatic hepcidin expression which may enhance iron toxicity and lead to disease progression and HCC development. The aim of the study is to investigate the role of hepcidin in HCV+ve liver cirrhosis patients in relation to disease progression and HCC development. Patients and methods: The study population consists of: 20 HCV+ve patients without HCC (HCV patients), 20 HCV+ve patients with HCC (HCV-HCC patients) and 10 controls. In addition to comprehensive clinical examination, they were subjected to laboratory check-up for albumin, bilirubin, PT %, ferritin, AFP and hepcidin. Ascitic fluid hepcidin was done for all patients. Results: There was a significant difference among HCV and HCV-HCC patients and controls with regard serum ferritin and hepcidin (P = 0.001 & 0.0001 respectively). Serum hepcidin of HCV and HCV-HCC patients were significantly lower than controls (P = 0.0001). Serum and ascitic fluid hepcidin of HCV-HCC patients was significantly lower than HCV patients (P = 0.01 & 0.02 respectively). Serum ferritin was significantly higher in HCV and HCV-HCC patients than controls (P = 0.001). Serum ferritin of HCV-HCC patients was significantly higher than HCV patients (P = 0.02). Ascitic fluid hepcidin was negatively correlated with Child-Pugh score in HCV (r=-0.55 & P=0.01) and HCV-HCC patients (r = -0.53 & P = 0.02). Ascitic fluid hepcidin was negatively correlated with bilirubin in HCV (r = -0.43 & P=0.04) and HCV-HCC patients (r = -0.47 & P=0.04). Ascitic fluid hepcidin was positively correlated with serum albumin in HCV (r = +0.44 & P=0.04) but there was no correlation in HCV-HCC patients (r =-0.1 & P=0.7). Conclusion: Low levels of hepcidin may be involved in the pathophysiologic mechanism of iron overload in patients with chronic HCV with and without HCC. Moreover, there is a positive relationship between hepcidin levels and synthetic liver function suggesting that uniform suppression of hepcidin may be linked to disease progression and HCC development. Further analysis is still required to evaluate its usefulness as a marker for early detection of HCC by serial measurement of hepcidin in blood and ascitic fluid.},
 year = {2014}
}

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TY  - JOUR
T1  - Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC
AU  - Ehab Abd El Atti
AU  - Alaa Dawood
AU  - Abdallah Said Essa
AU  - Bassam Mohamed Masoud
AU  - Enas Said Essa
AU  - Yasser El-Ghobashy
AU  - Ashraf Anas Zytoon
Y1  - 2014/01/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ejpm.20130103.13
DO  - 10.11648/j.ejpm.20130103.13
T2  - European Journal of Preventive Medicine
JF  - European Journal of Preventive Medicine
JO  - European Journal of Preventive Medicine
SP  - 63
EP  - 69
PB  - Science Publishing Group
SN  - 2330-8230
UR  - https://doi.org/10.11648/j.ejpm.20130103.13
AB  - Background & Aim: Chronic HCV infection suppresses hepatic hepcidin expression which may enhance iron toxicity and lead to disease progression and HCC development. The aim of the study is to investigate the role of hepcidin in HCV+ve liver cirrhosis patients in relation to disease progression and HCC development. Patients and methods: The study population consists of: 20 HCV+ve patients without HCC (HCV patients), 20 HCV+ve patients with HCC (HCV-HCC patients) and 10 controls. In addition to comprehensive clinical examination, they were subjected to laboratory check-up for albumin, bilirubin, PT %, ferritin, AFP and hepcidin. Ascitic fluid hepcidin was done for all patients. Results: There was a significant difference among HCV and HCV-HCC patients and controls with regard serum ferritin and hepcidin (P = 0.001 & 0.0001 respectively). Serum hepcidin of HCV and HCV-HCC patients were significantly lower than controls (P = 0.0001). Serum and ascitic fluid hepcidin of HCV-HCC patients was significantly lower than HCV patients (P = 0.01 & 0.02 respectively). Serum ferritin was significantly higher in HCV and HCV-HCC patients than controls (P = 0.001). Serum ferritin of HCV-HCC patients was significantly higher than HCV patients (P = 0.02). Ascitic fluid hepcidin was negatively correlated with Child-Pugh score in HCV (r=-0.55 & P=0.01) and HCV-HCC patients (r = -0.53 & P = 0.02). Ascitic fluid hepcidin was negatively correlated with bilirubin in HCV (r = -0.43 & P=0.04) and HCV-HCC patients (r = -0.47 & P=0.04). Ascitic fluid hepcidin was positively correlated with serum albumin in HCV (r = +0.44 & P=0.04) but there was no correlation in HCV-HCC patients (r =-0.1 & P=0.7). Conclusion: Low levels of hepcidin may be involved in the pathophysiologic mechanism of iron overload in patients with chronic HCV with and without HCC. Moreover, there is a positive relationship between hepcidin levels and synthetic liver function suggesting that uniform suppression of hepcidin may be linked to disease progression and HCC development. Further analysis is still required to evaluate its usefulness as a marker for early detection of HCC by serial measurement of hepcidin in blood and ascitic fluid.
VL  - 1
IS  - 3
ER  -

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Author Information

Ehab Abd El Atti

Internal Medicine Department, Faculty of Medicine, Menoufiya University, Egypt
Alaa Dawood

Internal Medicine Department, Faculty of Medicine, Menoufiya University, Egypt
Abdallah Said Essa

Tropical Medicine Department, Faculty of Medicine, Menoufiya University, Egypt
Bassam Mohamed Masoud

Tropical Medicine Department, Faculty of Medicine, Menoufiya University, Egypt
Enas Said Essa

Clinical Pathology Department, Faculty of Medicine, Menoufiya University, Egypt
Yasser El-Ghobashy

Biochemistry Department, Faculty of Medicine, Menoufiya University, Egypt
Ashraf Anas Zytoon

Radiology Department, Faculty of Medicine, Menoufiya University, Egypt

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APA Style

Ehab Abd El Atti, Alaa Dawood, Abdallah Said Essa, Bassam Mohamed Masoud, Enas Said Essa, et al. (2014). Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC. European Journal of Preventive Medicine, 1(3), 63-69. https://doi.org/10.11648/j.ejpm.20130103.13

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ACS Style

Ehab Abd El Atti; Alaa Dawood; Abdallah Said Essa; Bassam Mohamed Masoud; Enas Said Essa, et al. Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC. Eur. J. Prev. Med. 2014, 1(3), 63-69. doi: 10.11648/j.ejpm.20130103.13

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AMA Style

Ehab Abd El Atti, Alaa Dawood, Abdallah Said Essa, Bassam Mohamed Masoud, Enas Said Essa, et al. Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC. Eur J Prev Med. 2014;1(3):63-69. doi: 10.11648/j.ejpm.20130103.13

Copy | Download

@article{10.11648/j.ejpm.20130103.13,
  author = {Ehab Abd El Atti and Alaa Dawood and Abdallah Said Essa and Bassam Mohamed Masoud and Enas Said Essa and Yasser El-Ghobashy and Ashraf Anas Zytoon},
  title = {Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC},
  journal = {European Journal of Preventive Medicine},
  volume = {1},
  number = {3},
  pages = {63-69},
  doi = {10.11648/j.ejpm.20130103.13},
  url = {https://doi.org/10.11648/j.ejpm.20130103.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejpm.20130103.13},
  abstract = {Background & Aim: Chronic HCV infection suppresses hepatic hepcidin expression which may enhance iron toxicity and lead to disease progression and HCC development. The aim of the study is to investigate the role of hepcidin in HCV+ve liver cirrhosis patients in relation to disease progression and HCC development. Patients and methods: The study population consists of: 20 HCV+ve patients without HCC (HCV patients), 20 HCV+ve patients with HCC (HCV-HCC patients) and 10 controls. In addition to comprehensive clinical examination, they were subjected to laboratory check-up for albumin, bilirubin, PT %, ferritin, AFP and hepcidin. Ascitic fluid hepcidin was done for all patients. Results: There was a significant difference among HCV and HCV-HCC patients and controls with regard serum ferritin and hepcidin (P = 0.001 & 0.0001 respectively). Serum hepcidin of HCV and HCV-HCC patients were significantly lower than controls (P = 0.0001). Serum and ascitic fluid hepcidin of HCV-HCC patients was significantly lower than HCV patients (P = 0.01 & 0.02 respectively). Serum ferritin was significantly higher in HCV and HCV-HCC patients than controls (P = 0.001). Serum ferritin of HCV-HCC patients was significantly higher than HCV patients (P = 0.02). Ascitic fluid hepcidin was negatively correlated with Child-Pugh score in HCV (r=-0.55 & P=0.01) and HCV-HCC patients (r = -0.53 & P = 0.02). Ascitic fluid hepcidin was negatively correlated with bilirubin in HCV (r = -0.43 & P=0.04) and HCV-HCC patients (r = -0.47 & P=0.04). Ascitic fluid hepcidin was positively correlated with serum albumin in HCV (r = +0.44 & P=0.04) but there was no correlation in HCV-HCC patients (r =-0.1 & P=0.7). Conclusion: Low levels of hepcidin may be involved in the pathophysiologic mechanism of iron overload in patients with chronic HCV with and without HCC. Moreover, there is a positive relationship between hepcidin levels and synthetic liver function suggesting that uniform suppression of hepcidin may be linked to disease progression and HCC development. Further analysis is still required to evaluate its usefulness as a marker for early detection of HCC by serial measurement of hepcidin in blood and ascitic fluid.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Serum and Ascitic Fluid Hepcidin in HCV Positive Liver Cirrhosis with and without HCC
AU  - Ehab Abd El Atti
AU  - Alaa Dawood
AU  - Abdallah Said Essa
AU  - Bassam Mohamed Masoud
AU  - Enas Said Essa
AU  - Yasser El-Ghobashy
AU  - Ashraf Anas Zytoon
Y1  - 2014/01/10
PY  - 2014
N1  - https://doi.org/10.11648/j.ejpm.20130103.13
DO  - 10.11648/j.ejpm.20130103.13
T2  - European Journal of Preventive Medicine
JF  - European Journal of Preventive Medicine
JO  - European Journal of Preventive Medicine
SP  - 63
EP  - 69
PB  - Science Publishing Group
SN  - 2330-8230
UR  - https://doi.org/10.11648/j.ejpm.20130103.13
AB  - Background & Aim: Chronic HCV infection suppresses hepatic hepcidin expression which may enhance iron toxicity and lead to disease progression and HCC development. The aim of the study is to investigate the role of hepcidin in HCV+ve liver cirrhosis patients in relation to disease progression and HCC development. Patients and methods: The study population consists of: 20 HCV+ve patients without HCC (HCV patients), 20 HCV+ve patients with HCC (HCV-HCC patients) and 10 controls. In addition to comprehensive clinical examination, they were subjected to laboratory check-up for albumin, bilirubin, PT %, ferritin, AFP and hepcidin. Ascitic fluid hepcidin was done for all patients. Results: There was a significant difference among HCV and HCV-HCC patients and controls with regard serum ferritin and hepcidin (P = 0.001 & 0.0001 respectively). Serum hepcidin of HCV and HCV-HCC patients were significantly lower than controls (P = 0.0001). Serum and ascitic fluid hepcidin of HCV-HCC patients was significantly lower than HCV patients (P = 0.01 & 0.02 respectively). Serum ferritin was significantly higher in HCV and HCV-HCC patients than controls (P = 0.001). Serum ferritin of HCV-HCC patients was significantly higher than HCV patients (P = 0.02). Ascitic fluid hepcidin was negatively correlated with Child-Pugh score in HCV (r=-0.55 & P=0.01) and HCV-HCC patients (r = -0.53 & P = 0.02). Ascitic fluid hepcidin was negatively correlated with bilirubin in HCV (r = -0.43 & P=0.04) and HCV-HCC patients (r = -0.47 & P=0.04). Ascitic fluid hepcidin was positively correlated with serum albumin in HCV (r = +0.44 & P=0.04) but there was no correlation in HCV-HCC patients (r =-0.1 & P=0.7). Conclusion: Low levels of hepcidin may be involved in the pathophysiologic mechanism of iron overload in patients with chronic HCV with and without HCC. Moreover, there is a positive relationship between hepcidin levels and synthetic liver function suggesting that uniform suppression of hepcidin may be linked to disease progression and HCC development. Further analysis is still required to evaluate its usefulness as a marker for early detection of HCC by serial measurement of hepcidin in blood and ascitic fluid.
VL  - 1
IS  - 3
ER  -

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