The present study aimed to investigate the effect of the different types of solvents (water, ethanol and methanol) and extraction methods (ultrasound and maceration) on Punica granatum peels and to assess their antibacterial and antibiofilm activities. Our results revealed that the solvents and the extraction methods had a significant effect concerning the antimicrobial effects of P. granatum peels. The methanol extract produced by both the ultrasound and the maceration extraction techniques and the ethanol extract produced by the maceration extraction technique showed the most potent effect with the same bacteriostatic effect (MIC=25 mg/ml) on Staphylococcus epidermidis (CIP444). The antibacterial activities for all the P. granatum peels extracts were the same against E. coli (ATCC35218), with an MIC=25 mg/ml. The results showed that the methanol and the ethanol extracts produced by both the ultrasound and the maceration extraction techniques had an appreciable antibiofilm activity against S. epidermidis (CIP 444). These extracts showed the highest prevention capacity at a concentration of 12.5 mg/ml. In addition, the highest biofilm eradication activity was obtained using the ethanol extract with the ultrasound technique at a concentration of 25 mg/ml. We concluded that the P. granatum peels extracts have great potential as future natural antibacterial agents.
| Published in | Journal of Diseases and Medicinal Plants (Volume 4, Issue 6) |
| DOI | 10.11648/j.jdmp.20180406.11 |
| Page(s) | 133-141 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Punica granatum Peels, Antibacterial Activity, Antibiofilm Activity
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APA Style
Salam Nasreddine, Amale Mcheik, Mohammad Abdalla Al-Seblani, Hawraa Shahrour, Mariam Hammoud, et al. (2019). Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria. Journal of Diseases and Medicinal Plants, 4(6), 133-141. https://doi.org/10.11648/j.jdmp.20180406.11
ACS Style
Salam Nasreddine; Amale Mcheik; Mohammad Abdalla Al-Seblani; Hawraa Shahrour; Mariam Hammoud, et al. Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria. J. Dis. Med. Plants 2019, 4(6), 133-141. doi: 10.11648/j.jdmp.20180406.11
AMA Style
Salam Nasreddine, Amale Mcheik, Mohammad Abdalla Al-Seblani, Hawraa Shahrour, Mariam Hammoud, et al. Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria. J Dis Med Plants. 2019;4(6):133-141. doi: 10.11648/j.jdmp.20180406.11
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Download@article{10.11648/j.jdmp.20180406.11,
author = {Salam Nasreddine and Amale Mcheik and Mohammad Abdalla Al-Seblani and Hawraa Shahrour and Mariam Hammoud and Ali Chokr},
title = {Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria},
journal = {Journal of Diseases and Medicinal Plants},
volume = {4},
number = {6},
pages = {133-141},
doi = {10.11648/j.jdmp.20180406.11},
url = {https://doi.org/10.11648/j.jdmp.20180406.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180406.11},
abstract = {The present study aimed to investigate the effect of the different types of solvents (water, ethanol and methanol) and extraction methods (ultrasound and maceration) on Punica granatum peels and to assess their antibacterial and antibiofilm activities. Our results revealed that the solvents and the extraction methods had a significant effect concerning the antimicrobial effects of P. granatum peels. The methanol extract produced by both the ultrasound and the maceration extraction techniques and the ethanol extract produced by the maceration extraction technique showed the most potent effect with the same bacteriostatic effect (MIC=25 mg/ml) on Staphylococcus epidermidis (CIP444). The antibacterial activities for all the P. granatum peels extracts were the same against E. coli (ATCC35218), with an MIC=25 mg/ml. The results showed that the methanol and the ethanol extracts produced by both the ultrasound and the maceration extraction techniques had an appreciable antibiofilm activity against S. epidermidis (CIP 444). These extracts showed the highest prevention capacity at a concentration of 12.5 mg/ml. In addition, the highest biofilm eradication activity was obtained using the ethanol extract with the ultrasound technique at a concentration of 25 mg/ml. We concluded that the P. granatum peels extracts have great potential as future natural antibacterial agents.},
year = {2019}
}
TY - JOUR T1 - Influence of the Extractive Method of Punica granatum Peels in its Antibacterial and Antibiofilm Activities Against Gram-Negative and Gram-Positive Bacteria AU - Salam Nasreddine AU - Amale Mcheik AU - Mohammad Abdalla Al-Seblani AU - Hawraa Shahrour AU - Mariam Hammoud AU - Ali Chokr Y1 - 2019/01/22 PY - 2019 N1 - https://doi.org/10.11648/j.jdmp.20180406.11 DO - 10.11648/j.jdmp.20180406.11 T2 - Journal of Diseases and Medicinal Plants JF - Journal of Diseases and Medicinal Plants JO - Journal of Diseases and Medicinal Plants SP - 133 EP - 141 PB - Science Publishing Group SN - 2469-8210 UR - https://doi.org/10.11648/j.jdmp.20180406.11 AB - The present study aimed to investigate the effect of the different types of solvents (water, ethanol and methanol) and extraction methods (ultrasound and maceration) on Punica granatum peels and to assess their antibacterial and antibiofilm activities. Our results revealed that the solvents and the extraction methods had a significant effect concerning the antimicrobial effects of P. granatum peels. The methanol extract produced by both the ultrasound and the maceration extraction techniques and the ethanol extract produced by the maceration extraction technique showed the most potent effect with the same bacteriostatic effect (MIC=25 mg/ml) on Staphylococcus epidermidis (CIP444). The antibacterial activities for all the P. granatum peels extracts were the same against E. coli (ATCC35218), with an MIC=25 mg/ml. The results showed that the methanol and the ethanol extracts produced by both the ultrasound and the maceration extraction techniques had an appreciable antibiofilm activity against S. epidermidis (CIP 444). These extracts showed the highest prevention capacity at a concentration of 12.5 mg/ml. In addition, the highest biofilm eradication activity was obtained using the ethanol extract with the ultrasound technique at a concentration of 25 mg/ml. We concluded that the P. granatum peels extracts have great potential as future natural antibacterial agents. VL - 4 IS - 6 ER -
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