Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum <i>In Vitro</i> by Thiamin and Quinine

Atsuko Yamashita; Nana Shimamoto; Kyoko Morita; Hasumi Sugiyama; Shiho Tadakuma; Maki Kato; Mari Kimoto; Kazuo Toda; Masato Ota

doi:doi:10.11648/j.jfns.20180605.11

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine

Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Shiho Tadakuma, Maki Kato, Mari Kimoto, Kazuo Toda, Masato Ota

Published in Journal of Food and Nutrition Sciences (Volume 6, Issue 5)

Received: 5 July 2018 Accepted: 16 July 2018 Published: 22 October 2018

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Abstract

Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.

Published in	Journal of Food and Nutrition Sciences (Volume 6, Issue 5)
DOI	10.11648/j.jfns.20180605.11
Page(s)	115-122
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Thiamin, Quinine, Small Intestine, Mouse

References

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Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Shiho Tadakuma, et al. (2018). Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. Journal of Food and Nutrition Sciences, 6(5), 115-122. https://doi.org/10.11648/j.jfns.20180605.11

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Atsuko Yamashita; Nana Shimamoto; Kyoko Morita; Hasumi Sugiyama; Shiho Tadakuma, et al. Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. J. Food Nutr. Sci. 2018, 6(5), 115-122. doi: 10.11648/j.jfns.20180605.11

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AMA Style

Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Shiho Tadakuma, et al. Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. J Food Nutr Sci. 2018;6(5):115-122. doi: 10.11648/j.jfns.20180605.11

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@article{10.11648/j.jfns.20180605.11,
  author = {Atsuko Yamashita and Nana Shimamoto and Kyoko Morita and Hasumi Sugiyama and Shiho Tadakuma and Maki Kato and Mari Kimoto and Kazuo Toda and Masato Ota},
  title = {Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {6},
  number = {5},
  pages = {115-122},
  doi = {10.11648/j.jfns.20180605.11},
  url = {https://doi.org/10.11648/j.jfns.20180605.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20180605.11},
  abstract = {Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.},
 year = {2018}
}

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TY  - JOUR
T1  - Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine
AU  - Atsuko Yamashita
AU  - Nana Shimamoto
AU  - Kyoko Morita
AU  - Hasumi Sugiyama
AU  - Shiho Tadakuma
AU  - Maki Kato
AU  - Mari Kimoto
AU  - Kazuo Toda
AU  - Masato Ota
Y1  - 2018/10/22
PY  - 2018
N1  - https://doi.org/10.11648/j.jfns.20180605.11
DO  - 10.11648/j.jfns.20180605.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 115
EP  - 122
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20180605.11
AB  - Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.
VL  - 6
IS  - 5
ER  -

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Author Information

Atsuko Yamashita

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan
Nana Shimamoto

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan
Kyoko Morita

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan
Hasumi Sugiyama

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan
Shiho Tadakuma

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan
Maki Kato

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan
Mari Kimoto

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan
Kazuo Toda

Integrative Sensory Physiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
Masato Ota

Laboratory of Anatomy, Physiology, and Food Biological Science, Department of Food and Nutrition, Japan Women's University, Tokyo, Japan

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APA Style

Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Shiho Tadakuma, et al. (2018). Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. Journal of Food and Nutrition Sciences, 6(5), 115-122. https://doi.org/10.11648/j.jfns.20180605.11

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ACS Style

Atsuko Yamashita; Nana Shimamoto; Kyoko Morita; Hasumi Sugiyama; Shiho Tadakuma, et al. Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. J. Food Nutr. Sci. 2018, 6(5), 115-122. doi: 10.11648/j.jfns.20180605.11

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AMA Style

Atsuko Yamashita, Nana Shimamoto, Kyoko Morita, Hasumi Sugiyama, Shiho Tadakuma, et al. Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine. J Food Nutr Sci. 2018;6(5):115-122. doi: 10.11648/j.jfns.20180605.11

Copy | Download

@article{10.11648/j.jfns.20180605.11,
  author = {Atsuko Yamashita and Nana Shimamoto and Kyoko Morita and Hasumi Sugiyama and Shiho Tadakuma and Maki Kato and Mari Kimoto and Kazuo Toda and Masato Ota},
  title = {Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine},
  journal = {Journal of Food and Nutrition Sciences},
  volume = {6},
  number = {5},
  pages = {115-122},
  doi = {10.11648/j.jfns.20180605.11},
  url = {https://doi.org/10.11648/j.jfns.20180605.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.20180605.11},
  abstract = {Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum In Vitro by Thiamin and Quinine
AU  - Atsuko Yamashita
AU  - Nana Shimamoto
AU  - Kyoko Morita
AU  - Hasumi Sugiyama
AU  - Shiho Tadakuma
AU  - Maki Kato
AU  - Mari Kimoto
AU  - Kazuo Toda
AU  - Masato Ota
Y1  - 2018/10/22
PY  - 2018
N1  - https://doi.org/10.11648/j.jfns.20180605.11
DO  - 10.11648/j.jfns.20180605.11
T2  - Journal of Food and Nutrition Sciences
JF  - Journal of Food and Nutrition Sciences
JO  - Journal of Food and Nutrition Sciences
SP  - 115
EP  - 122
PB  - Science Publishing Group
SN  - 2330-7293
UR  - https://doi.org/10.11648/j.jfns.20180605.11
AB  - Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Qui-induced inhibition of the contraction in the jejunum.
VL  - 6
IS  - 5
ER  -

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