To elucidate the basis of protective immunity in T and B cell deficient rag1-/- mutant zebrafish, we conducted microarray analysis of 15,617 genes from rag1-/- mutant zebrafish 48 hours after a primary response and 48 hours after a secondary response. Following primary exposure, the highest fold expression differences (3.8 to 4.95) were genes for serum amyloid A, chemokine CCL-C5a (CCL-19a), signal transducer and activator of transcription (STAT) 1b, interferon regulatory factor 11, and myxovirus resistance A. Strong induction of these genes demonstrated that primary immune responses and innate immune cells were not impaired in T and B cell deficient mutant zebrafish. Following bacterial re-exposure, the highest fold expression differences (2 to 3 fold) were in chemokine CCL-C5a (CCL-19a), myomegalin, bone morphogenetic protein 4, and relaxin 3a. These genes are involved in the immune response and cell proliferation. Genes for cell receptor activation and signal transduction, cell proliferation and cytotoxic functions were also up-regulated. These findings suggest receptor activation and expansion of a cell population. Increased ifnγ expression at 48 hpi was associated with both primary and secondary immune responses.
| Published in | International Journal of Immunology (Volume 5, Issue 2) |
| DOI | 10.11648/j.iji.20170502.11 |
| Page(s) | 20-36 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
rag1-/- Mutant Zebrafish, Edwardsiella ictaluri, Protective Immunity, Transcriptome, Cell-Mediated Immunity
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APA Style
Aparna Krishnavajhala, Preeti J. Muire, Larry Hanson, Henry Wan, Fiona McCarthy, et al. (2017). Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish. International Journal of Immunology, 5(2), 20-36. https://doi.org/10.11648/j.iji.20170502.11
ACS Style
Aparna Krishnavajhala; Preeti J. Muire; Larry Hanson; Henry Wan; Fiona McCarthy, et al. Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish. Int. J. Immunol. 2017, 5(2), 20-36. doi: 10.11648/j.iji.20170502.11
AMA Style
Aparna Krishnavajhala, Preeti J. Muire, Larry Hanson, Henry Wan, Fiona McCarthy, et al. Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish. Int J Immunol. 2017;5(2):20-36. doi: 10.11648/j.iji.20170502.11
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Download@article{10.11648/j.iji.20170502.11,
author = {Aparna Krishnavajhala and Preeti J. Muire and Larry Hanson and Henry Wan and Fiona McCarthy and Alan Zhou and Lora Petrie-Hanson},
title = {Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish},
journal = {International Journal of Immunology},
volume = {5},
number = {2},
pages = {20-36},
doi = {10.11648/j.iji.20170502.11},
url = {https://doi.org/10.11648/j.iji.20170502.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20170502.11},
abstract = {To elucidate the basis of protective immunity in T and B cell deficient rag1-/- mutant zebrafish, we conducted microarray analysis of 15,617 genes from rag1-/- mutant zebrafish 48 hours after a primary response and 48 hours after a secondary response. Following primary exposure, the highest fold expression differences (3.8 to 4.95) were genes for serum amyloid A, chemokine CCL-C5a (CCL-19a), signal transducer and activator of transcription (STAT) 1b, interferon regulatory factor 11, and myxovirus resistance A. Strong induction of these genes demonstrated that primary immune responses and innate immune cells were not impaired in T and B cell deficient mutant zebrafish. Following bacterial re-exposure, the highest fold expression differences (2 to 3 fold) were in chemokine CCL-C5a (CCL-19a), myomegalin, bone morphogenetic protein 4, and relaxin 3a. These genes are involved in the immune response and cell proliferation. Genes for cell receptor activation and signal transduction, cell proliferation and cytotoxic functions were also up-regulated. These findings suggest receptor activation and expansion of a cell population. Increased ifnγ expression at 48 hpi was associated with both primary and secondary immune responses.},
year = {2017}
}
TY - JOUR T1 - Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish AU - Aparna Krishnavajhala AU - Preeti J. Muire AU - Larry Hanson AU - Henry Wan AU - Fiona McCarthy AU - Alan Zhou AU - Lora Petrie-Hanson Y1 - 2017/03/25 PY - 2017 N1 - https://doi.org/10.11648/j.iji.20170502.11 DO - 10.11648/j.iji.20170502.11 T2 - International Journal of Immunology JF - International Journal of Immunology JO - International Journal of Immunology SP - 20 EP - 36 PB - Science Publishing Group SN - 2329-1753 UR - https://doi.org/10.11648/j.iji.20170502.11 AB - To elucidate the basis of protective immunity in T and B cell deficient rag1-/- mutant zebrafish, we conducted microarray analysis of 15,617 genes from rag1-/- mutant zebrafish 48 hours after a primary response and 48 hours after a secondary response. Following primary exposure, the highest fold expression differences (3.8 to 4.95) were genes for serum amyloid A, chemokine CCL-C5a (CCL-19a), signal transducer and activator of transcription (STAT) 1b, interferon regulatory factor 11, and myxovirus resistance A. Strong induction of these genes demonstrated that primary immune responses and innate immune cells were not impaired in T and B cell deficient mutant zebrafish. Following bacterial re-exposure, the highest fold expression differences (2 to 3 fold) were in chemokine CCL-C5a (CCL-19a), myomegalin, bone morphogenetic protein 4, and relaxin 3a. These genes are involved in the immune response and cell proliferation. Genes for cell receptor activation and signal transduction, cell proliferation and cytotoxic functions were also up-regulated. These findings suggest receptor activation and expansion of a cell population. Increased ifnγ expression at 48 hpi was associated with both primary and secondary immune responses. VL - 5 IS - 2 ER -
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