Carbon and nitrogen metabolism pathways are regulated by complex mechanisms in order to optimize growth and development of plants. This study was conducted to contribute to the determination of roles of some key enzymes of carbon and nitrogen metabolism in sorghum roots. Sorghum were grown under nitrate (NO3-), ammonium (NH 4+) or nitrate combined to ammonium at different concentrations. Growth parameters and protein content were evaluated. In addition, we analyzed key enzymes activities involved in nitrogen and carbon metabolism such as: nitrate reductase (NR), glutamine synthetase (GS), aspartate aminotransferase (AAT) and phosphoenolpyruvate carboxylase (PEPC). At high nitrogen levels (ammonium or nitrate), the sorghum roots showed an increase in biomass accompanied by an increase in protein content. Likewise, high concentrations induced accumulation of the non-photosynthetic isoform of phosphoenolpyruvate carboxylase. Glutamine synthetase and aspartate aminotransferase activities were also increased, especially at high levels of ammonium. These results showed that sorghum plants have an efficient system for nitrogen assimilation in their roots, allowing this plant to tolerate excessive concentration of this nutrient.
| Published in | Plant (Volume 4, Issue 6) |
| DOI | 10.11648/j.plant.20160406.14 |
| Page(s) | 71-77 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Sorghum, Nitrogen Stress, Nitrogen Metabolism, Ammonium, Nitrate
| [1] | Epstein, E., Bloom, A. J., (2005): Mineral Nutrition of Plants: Principles and Perspectives, 2nd edition. Sinauer, Sunderland, MA. |
| [2] | Valadier M. H., A. Yoshida, O. Grandjean, H. Morin, J. Kronenberger, S. Boutet, A. Raballand, T. Hase, T. Yoneyama and A. Suzuki, (2008): Implication of the glutamine synthetase/glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves. F. E. B. S. Journal, 275: 3193-3206. |
| [3] | Nimmo H. G. (2003): Control of the phosphorylation of phosphoenolpyruvate carboxylase in higher plants, Archives of Biochemistry and Biophysics, 414: 189–196. |
| [4] | Izui K., Matsumura H., Furumoto T., Kai Y. (2004): phosphoenolpyruvate carboxylase: a new era of structural biology. Annual Review of Plant Biology, 55: 69–84. |
| [5] | O’Leary B., Park J. and Plaxton W. C. (2011): The remarkable diversity of plant PEPC (phosphoenolpyruvate carboxylase): recent insights into the physiological functions and post-translational controls of non-photosynthetic PEPCs. Biochemical Journal, 436: 15–34. |
| [6] | Agrama, H. A. and M. R. Tuinstra. (2003): Phylogenetic diversity and relationships among sorghum accessions using SSRs and RAPDs. African. J. Biotechnol., 2 (10): 334-340. |
| [7] | Nhiri M., Bakrim N., Pacquit V., Hachimi-Messouak ZE., Osuna L., Vidal J., (1998): Calcium-dependent and –independent phosphoenolpyruvate carboxylase kinases in sorghum leaves: further evidence for the involvment of the calcium-independent protein kinase in the in-situ regulatory phosphorylation of C4 phosphoenolpyruvate carboxylase. Plant Cell Physiol., 39: 241-6. |
| [8] | El Omari R., Rueda-López M., Avila C., Crespillo R., Nhiri N. and M. Cánovas F., (2010): Ammonium tolerance and the regulation of two cytosolic glutamine synthetases in the roots of sorghum. Functional Plant Biology, 37: 55–63. |
| [9] | Arnon D. I. (1938): Microelements in culture solution experiments with higher plants. American Journal of Botany, 25: 322–325. |
| [10] | Shapiro B. M. and Stadtman E. R. (1970): Glutamine synthetase (Escherichia coli). In H. Tabor and W. C. Tabor (eds.), Methods in Enzymology, Academic Press, New York and London Vol: 17A 910–912. |
| [11] | Kaiser J. J, O. A. H Lewis, (1984): Nitrate reductase and glutamine synthetase activity in leaves and roots of nitrate-fed Helianthus annuus L. Plant and Soil. 70: 127-30. |
| [12] | Bradford M. M. (1976): A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem., 72: 248–254. |
| [13] | Mahmood, T., Kaiser, W. M., (2003): Growth and solute composition of the salt-tolerant kallar grass [Leptochloa fusca, (L.) Kunth] as affected by nitrogen source. Plant and Soil, 252: 359–366. |
| [14] | Abbasi M. K., Tahir M. M., Rahim N., (2013): Effect of N fertilizer source and timing on yield and N use efficiency of rainfed maize (Zea mays L.) in Kashmir–Pakistan Geoderma Volumes 195–196, Pages 87–93. |
| [15] | Lasa BS, Frechilla PM, Aparicio-Tejo C, Lamsfus (2002): Role of glutamate dehydrogenase and phosphoenolpyruvate carboxylase activity in ammonium nutrition tolerance in roots, Plant Physiol. Biochem., 40: 969–976. |
| [16] | Arnozis P. A., Nelemans J. A. & Findenegg G. R. (1988): Phosphoenolpyruvate carboxylase activity in plants grown with eitherNO3- or NH4+ as inorganic nitrogen source. Journal of Plant Physiology, 132: 23–27. |
| [17] | Viktor A., Cramer M. D. (2005): The influence of root assimilated inorganic carbon on nitrogen acquisition/assimilation and carbon partitioning. New Phytol, 165: 157–69. |
| [18] | Setién I, Vega-Mas I, Celestino N, Calleja-Cervantes M. E., González-Murua C., Estavillo J. M., González-Moro M. B., (2014): Root phosphoenolpyruvate carboxylase and NAD-malic enzymes activity increase the ammonium-assimilating capacity in tomato. Journal of Plant Physiology, 171, Issue 5, 1: 49–63. |
| [19] | Viktor, A and Cramer M. D. (2005): The influence of root assimilated inorganic carbon on nitrogen acquisition/assimilation and carbon partitioning. New Phytol., 165: 157–169. |
| [20] | Sugiharto, B., and Sugiyama, T. (1992): Effects of nitrate and ammonium on gene expression of phosphoénolpyruvate carboxylase and nitrogen metabolism in maize leaf tissue during recorvery from nitrogen stress. Plant Physiol., 98: 14031408. |
| [21] | El Omari R., Nhiri M. (2015): Effect of High Levels of Ammonium or Nitrate on Growth and Nitrogen Metabolism in Roots and Leaves of Sorghum (Sorghum sudangrass) Plants. American-Eurasian J. Agric. & Environ. Sci., 15: 1860-1867. |
| [22] | Britto, D. T., and H. J. Kronzucker. (2005): Nitrogen acquisition, PEP carboxylase, and cellular pH homeostasis: new views on old paradigms. Plant, Cell and Environment 28: 1396–1409. |
| [23] | Robin P, D Blayac, L Salsac (1979): Influence of nitric alimentation on nitrite content and nitrate reductase activity of maize roots and leaves. Physiol Veg., 17: 55-66. (French). |
| [24] | Atkins CA, Pate JS, Griftiths GJ. White ST. (1980): Economy or carbon and nitrogen in nodulated and nonnodulated (NO3- grown) cowpea (Vigna unguiculata (L.) Walp). Plant Physiology, 66: 978-983. |
| [25] | Fan X. H., Tang C., & Rengel Z. (2002): Nitrate uptake, nitrate reductase distribution and their relation to proton release in five nodulated grain legumes. Annals of botany, 90: 315-323. |
| [26] | Munzarova E., Lorenzen B., Brix H., Vojtiskova L., & Votrubova O. (2006): Effect of NH 4+/NO 3− availability on nitrate reductase activity and nitrogen accumulation in wetland helophytes Phragmites australis and Glyceria maxima. Environmental and Experimental Botany, 55: 49-60. |
| [27] | Oaks A., Aslam M., Boesel I., (1977): Ammonium and amino acids as regulators of nitrate reductase in corn roots. Plant Physiol., 59: 391-394. |
| [28] | Hirel B, Bouet C, King B, Layzell D, Jacobs F, Verma DPS (1987): Glutamine synthetase genes are regulated by ammonia provided externally or symbiotic nitrogen fixation. EMBO J., 6: 1167-1171. |
| [29] | De la Torre F. De Santis. Suarez MF. Crespillo R. Canovas FM. (2006): Identification and functional analysis of a prokaryotic-type aspartate aminotransférase: implications for plant amino acid metabolism. Plant J., 46: 414-425. |
| [30] | Wilkie S. E. and Warren M. J. (1998): Recombinant expression, purification, and characterization of three isoenzymes of aspartate aminotranferase from Arabidopsis thaliana. Protein Expr. Purif., 12: 381–389. |
| [31] | Coruzzi G. M. (2003) Primary N-assimilation into amino acids in Arabidopsis. In The Arabidopsis Book (Somerville, C. R. and Meyerowitz, E. M., eds). Rockville, MD: American Society of Plant Biologists, pp. 1–17. |
| [32] | Wadsworth, G. J. (1997): The plant aspartate aminotransferase gene family. Physiol. Plantarum, 100: 998-1006. |
APA Style
Reda Ben Mrid, Redouane El Omari, Mohamed Nhiri. (2016). Effect of Nitrogen Source and Concentration on Growth and Activity of Nitrogen Assimilation Enzymes in Roots of a Moroccan Sorghum Ecotype. Plant, 4(6), 71-77. https://doi.org/10.11648/j.plant.20160406.14
ACS Style
Reda Ben Mrid; Redouane El Omari; Mohamed Nhiri. Effect of Nitrogen Source and Concentration on Growth and Activity of Nitrogen Assimilation Enzymes in Roots of a Moroccan Sorghum Ecotype. Plant. 2016, 4(6), 71-77. doi: 10.11648/j.plant.20160406.14
AMA Style
Reda Ben Mrid, Redouane El Omari, Mohamed Nhiri. Effect of Nitrogen Source and Concentration on Growth and Activity of Nitrogen Assimilation Enzymes in Roots of a Moroccan Sorghum Ecotype. Plant. 2016;4(6):71-77. doi: 10.11648/j.plant.20160406.14
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Download@article{10.11648/j.plant.20160406.14,
author = {Reda Ben Mrid and Redouane El Omari and Mohamed Nhiri},
title = {Effect of Nitrogen Source and Concentration on Growth and Activity of Nitrogen Assimilation Enzymes in Roots of a Moroccan Sorghum Ecotype},
journal = {Plant},
volume = {4},
number = {6},
pages = {71-77},
doi = {10.11648/j.plant.20160406.14},
url = {https://doi.org/10.11648/j.plant.20160406.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20160406.14},
abstract = {Carbon and nitrogen metabolism pathways are regulated by complex mechanisms in order to optimize growth and development of plants. This study was conducted to contribute to the determination of roles of some key enzymes of carbon and nitrogen metabolism in sorghum roots. Sorghum were grown under nitrate (NO3-), ammonium (NH 4+) or nitrate combined to ammonium at different concentrations. Growth parameters and protein content were evaluated. In addition, we analyzed key enzymes activities involved in nitrogen and carbon metabolism such as: nitrate reductase (NR), glutamine synthetase (GS), aspartate aminotransferase (AAT) and phosphoenolpyruvate carboxylase (PEPC). At high nitrogen levels (ammonium or nitrate), the sorghum roots showed an increase in biomass accompanied by an increase in protein content. Likewise, high concentrations induced accumulation of the non-photosynthetic isoform of phosphoenolpyruvate carboxylase. Glutamine synthetase and aspartate aminotransferase activities were also increased, especially at high levels of ammonium. These results showed that sorghum plants have an efficient system for nitrogen assimilation in their roots, allowing this plant to tolerate excessive concentration of this nutrient.},
year = {2016}
}
TY - JOUR T1 - Effect of Nitrogen Source and Concentration on Growth and Activity of Nitrogen Assimilation Enzymes in Roots of a Moroccan Sorghum Ecotype AU - Reda Ben Mrid AU - Redouane El Omari AU - Mohamed Nhiri Y1 - 2016/10/31 PY - 2016 N1 - https://doi.org/10.11648/j.plant.20160406.14 DO - 10.11648/j.plant.20160406.14 T2 - Plant JF - Plant JO - Plant SP - 71 EP - 77 PB - Science Publishing Group SN - 2331-0677 UR - https://doi.org/10.11648/j.plant.20160406.14 AB - Carbon and nitrogen metabolism pathways are regulated by complex mechanisms in order to optimize growth and development of plants. This study was conducted to contribute to the determination of roles of some key enzymes of carbon and nitrogen metabolism in sorghum roots. Sorghum were grown under nitrate (NO3-), ammonium (NH 4+) or nitrate combined to ammonium at different concentrations. Growth parameters and protein content were evaluated. In addition, we analyzed key enzymes activities involved in nitrogen and carbon metabolism such as: nitrate reductase (NR), glutamine synthetase (GS), aspartate aminotransferase (AAT) and phosphoenolpyruvate carboxylase (PEPC). At high nitrogen levels (ammonium or nitrate), the sorghum roots showed an increase in biomass accompanied by an increase in protein content. Likewise, high concentrations induced accumulation of the non-photosynthetic isoform of phosphoenolpyruvate carboxylase. Glutamine synthetase and aspartate aminotransferase activities were also increased, especially at high levels of ammonium. These results showed that sorghum plants have an efficient system for nitrogen assimilation in their roots, allowing this plant to tolerate excessive concentration of this nutrient. VL - 4 IS - 6 ER -
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